(P2025 - 1S) BIOLOGÍA MOLECULAR ATarea Autónoma Unidad 1 Sem 5
Realice un calculo de mezcla de reactivos para PCR con los siguientes datos para cada grupo
La concentración requerida esta en la segunda fila de cada estudiante.
La concentración requerida para el tampón y para el MgCl2 lo pone cada estudiante
El numero de pacientes es de 7
|
Grupo |
Tampón |
MgCl2 |
dNTPs |
P1 |
P2 |
Taq pol |
ADN muestra |
Vol / tubo |
|
1 |
5X |
25 mM |
100um |
6pM/ul |
7pM/ul |
5UI/ul |
20ng/ul |
|
|
|
|
10um |
50 pM |
60 pM |
1 UI |
110ng |
50 ul |
|
|
2 |
10X |
50 mM |
200um |
8pM/ul |
5pM/ul |
5UI/ul |
21ng/ul |
|
|
|
|
14um |
42 pM |
50 pM |
2 UI |
120ng |
80 ul |
|
|
3 |
5X |
25 mM |
120um |
7pM/ul |
6pM/ul |
5UI/ul |
22ng/ul |
|
|
|
|
11um |
60 pM |
63 pM |
2 UI |
130ng |
100 ul |
|
|
4 |
10X |
50 mM |
100um |
9pM/ul |
9pM/ul |
5UI/ul |
23ng/ul |
|
|
|
|
13um |
80 pM |
60 pM |
2 UI |
135ng |
120 ul |
|
|
5 |
5X |
25 mM |
150um |
12pM/ul |
10pM/ul |
5UI/ul |
30ng/ul |
|
|
|
|
15um |
50 pM |
60 pM |
1 UI |
200ng |
50 ul |
|
|
6 |
10X |
50 mM |
110um |
10pM/ul |
8pM/ul |
5UI/ul |
21ng/ul |
|
|
|
|
8um |
70 pM |
60 pM |
1 UI |
120ng |
80 ul |
|
|
7 |
5X |
25 mM |
100um |
5pM/ul |
11pM/ul |
5UI/ul |
22ng/ul |
|
|
|
|
10um |
90 pM |
60 pM |
2 UI |
130ng |
100 ul |
|
|
8 |
10X |
50 mM |
200um |
11pM/ul |
12pM/ul |
5UI/ul |
23ng/ul |
|
|
|
|
14um |
85 pM |
60 pM |
1,5 UI |
135ng |
120 ul |
|
|
9 |
5X |
25 mM |
120um |
15pM/ul |
13pM/ul |
5UI/ul |
20ng/ul |
|
|
|
|
11um |
65 pM |
60 pM |
1 UI |
110ng |
50 ul |
|
|
10 |
10X |
50 mM |
100um |
14pM/ul |
8pM/ul |
5UI/ul |
21ng/ul |
|
|
|
|
13um |
45 pM |
60 pM |
1 UI |
120ng |
50 ul |
|
|
11 |
5X |
25 mM |
150um |
13pM/ul |
10pM/ul |
5UI/ul |
22ng/ul |
|
|
|
|
15um |
75 pM |
60 pM |
2 UI |
130ng |
80 ul |
|
|
12 |
10X |
50 mM |
110um |
16pM/ul |
14pM/ul |
5UI/ul |
23ng/ul |
|
|
|
|
8um |
64 pM |
60 pM |
1,5 UI |
135ng |
100 ul |
|
|
13 |
5X |
25 mM |
100um |
4pM/ul |
6pM/ul |
5UI/ul |
20ng/ul |
|
|
|
|
10um |
36 pM |
60 pM |
1 UI |
110ng |
120 ul |
|
|
14 |
10X |
50 mM |
200um |
6pM/ul |
7pM/ul |
5UI/ul |
21ng/ul |
|
|
|
|
14um |
50 pM |
60 pM |
2 UI |
120ng |
50 ul |
- 30 de mayo de 2025, 22:16